Tuesday, March 19, 2013

About Regeneration of Plants.


ARTIFICIAL REGENERATION                                            Compiled by-Nripesh Awasthi        
                                                                                                                              
“Artificial regeneration is defined as renewal of forest crops by sowing, planting or other artificial method. It also refers to the crops so obtained.” Normally such crops is called plantation.
OBJECT OF A.R.
1.      Reforestation- restocking of felled or other cleared woodland by artificial means. The place has vegetation before.
Objective:
·         To supplement natural regeneration.
·         To give up natural regeneration in favor of artificial regeneration.
·         To restock forest destroyed by fire or other biotic factors.
·         To change the composition of crops.
·         To introduce exotics.              
2.      Afforestation- establishment of forest by artificial means from which forest vegetation has long been absent or always been absent.
Objectives:
·         To increase the production of timber.
·         To increase the production of fuel and small timber.
·         Improvement of agro ecosystem
·         Moderation of climate.
·         Soil conservation
·         Protection of catchment of rivers.
·         Increasing natural beauty of landscape.
Artificial regeneration by:
1.      Seed
2.      Vegetation method
1.      A.R. from seed
a)      Direct sowing
Ø  Applied to areas, where soil operation is difficulties or soil tillage is difficult due to risk of landslide or cliff-terrain.
Ø  Fine seed mixed with sand and directly sowing can be done.
Eg: utis, khair, etc.
b)      Seedling plantation
Ø  Seedling are raised in nursery and transplanted into field during monsoon using polypot size 3*7.
Ø  Seedling such as bare rooted plant, large ball rooted seedling or stump (root-shoot cutting) are used in seedling plantation.
Ø  Survival rate of bare rooted plant is low but easy and cheap for handling and transportation to the site, eg: utis, Cryptomeria japonica.
Ø  Laborious and costly in handling and transporting in case of large ball rooted plant.
Ø  Stumps are being used in Nepal mainly for Dalbergia sissoo, Tectona grandis, Cassia siama, etc.
2.      A.R. from vegetative parts:
Regeneration from somatic parts of plant such as root, stem, leaves and bud accomplished by asexual reproduction is called regeneration from vegetative parts. Crops remain true to their parent and mature early. ie; Budding, cutting, coppicing, cell tissue culture, grafting, layering, root-sucker, etc.
ADVANTAGES
1.      It is useful to those plant that don’t produce viable seeds and difficult to regenerate through seed.
2.      It produces large number of population of clones in shortest time and cheaper.
3.      It preserves purity, resistance and good quality of races indefinitely.
4.      It maintains genetic uniformity to parental plants.
A)     BUDDING
In it, a bud with some portion of bark of a genetically superior plant is grafted on a inferior plant, so that it may produce new shoot when shoot of stock is cut off.
Eg: apple, peach, etc.
B)     CUTTING
It is cut piece of root, stem and leaves which is planted in nurseries. In it, root promotive hormones are added such as IBA, NAA, etc. It is used when seeds are not available of a species or difficult to raise from to seed. It reduces time for growing in nurseries and maintains genetic ideal plants.
Stem cutting- rose, sugarcane, bougainvillea, etc.
Root cutting- apple, lemon, etc 
Leaf cutting- snake plant (sansevieria)
C)     COPPICING
It is defined as shoot arise from the adventitious buds at the base of woody plants that has been cut near the ground or burnt back. It may be seedling coppice and stool coppice. It is good for highly sprouting deciduous broad leaved species aiming for production of small sized timber and fuelwood. It depends on following factors.
1.      Species
Ø  Strongly coppice: sal, khair, salix, Syzigium, Morus alba, Tectona grandis, Dalbergia spp, etc.
Ø  Coppice fairly: okhar, asna, terminalia spp, etc.
Ø  Coppice badly: Adina cardifolia, Bombax ceiba, Casuariana equisettifolia, etc.
Ø  Donot coppice: coniferous plants.
2.      Age of trees: younger trees coppices rapidly.
3.      Season of coppicing: little before the growth starts in spring.
4.      Height of stumps: 15-25 cm being very suitable.
D)     CELL TISSUE CULTURE
It is also called micro propagation. It includes propagation of plants by culturing the cells, tissues, and organs especially on artificial medium in suitable containers under controlled environment condition which is called tissue culture. The part which is cultured is called explants. This capacity to generate whole plant from any cell or explants is called cellular totipotency.
E)      GRAFTING
It is technique of connecting two parts; usually root system and shoot system of two different plants in a such a way that they unite and later develop into composite plants. In it, scion and stock are united by callus and cambium- cambium union is necessary. Common technique are crown grafting, side grafting, approach grafting, tongue grafting, wedge grafting, etc. Generally cleft grafting is useful in forestry.
Eg: pine, mango, rubber plant, citrus plant, plum, peach,etc
F)      LAYERING
It is a type of rooting cutting in which adventitious roots are induced to develop on a stem while it is still attached to the plant. It is carried out on one year old basal shoot branches commonly during early spring or early rainy season.
Ø  Air layering/gootee:  It is practiced in tropical and subtropical trees and shrubs. In it, 3-5 cm long ring of bark is removed from basal region of healthy and woody branch and covered by grafting clay (1 part cowdung or 1 part cut hay or mosses), rooting hormone wrapped in polythene.eg; litchi, pomegranate,etc.
Ø  Mound layering/ soil layering: In it, branch is pruned, lowered part of it covered with soil. Then a number of shoot is developed and rooted shoot are separated for planting.eg; apple, pear, etc.
G)     ROOT SUCKER
It is process in which root is partially or wholly cut to produce shoot is called root sucker propagation. Eg; sissoo, simal, etc.

Reference:
-Khanna L.S (2011). Principles and Practice of Silviculture. 
-Various books and magazines.